| 质粒名称: | pSIV-D3psi/delta env/delta Vif/delta Vpr |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 132928 |
| 存储实验室: | Jeremy Luban |
|
载体骨架基本信息 |
|
| 载体名称: | pUC57-mini |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 9787 bp |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 30°C |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Gag, pol, and vpx |
| 物种来源: | SIV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSIV-D3psi/delta env/delta Vif/delta Vpr was a gift from Jeremy Luban (Addgene plasmid # 132928 ; http://n2t.net/addgene:132928 ; RRID:Addgene_132928) |
| 参考文献部分: | Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha. Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C, Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J. Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 10.1038/s41564-019-0592-5. Epub 2019 Oct 21. 10.1038/s41564-019-0592-5 PubMed 31636416 |
买家导航
pSIV-D3psi/delta env/delta Vif/delta Vpr载体质粒基本信息
pSIV-D3psi/delta env/delta Vif/delta Vpr质粒图谱载体图谱和pSIV-D3psi/delta env/delta Vif/delta Vpr载体序列质粒序列多克隆位点信息
pSIV-D3psi/delta env/delta Vif/delta Vpr质粒载体简介
pSIV-D3psi/delta env/delta Vif/delta Vpr质粒序列载体序列
LOCUS pSIV-D3psi/delta env/delta Vif/delta Vpr 9787 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Production of SIV-VLPs containing Vpx protein.
ACCESSION .
VERSION .
KEYWORDS pSIV-D3psi/delta env/delta Vif/delta Vpr
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9787)
AUTHORS Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C,
Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
TITLE Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.
JOURNAL Nat Microbiol. 2019 Dec;4(12):2044-2051. doi:
10.1038/s41564-019-0592-5. Epub 2019 Oct 21.
PUBMED 31636416
REFERENCE 2 (bases 1 to 9787)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9787
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
polyA_signal 7679..7813
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7716..7735)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7770..7789
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 7865..7969
/gene="bla"
/label=AmpR promoter
CDS 7970..8830
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8188..8207)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9001..9589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9490..9509
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9675..9691
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9675..9691
/label=pBluescriptSK
/note="For pBluescript vector"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
661 ccagcctccg gtcgaggccg caagcttggc ctccggttgc aggtaagtgc aacacaaaaa
721 agaaatagct gtcttgttat ccaggaaggg ataataagat agagtgggag atgggcgcga
781 gaaactccgt cttgtcaggg aagaaagcag atgaattaga aaaaattagg ctacgacccg
841 gcggaaagaa aaagtacatg ttgaagcatg tagtatgggc agcaaatgaa ttagatagat
901 ttggattagc agaaagcctg ttggagaaca aagaaggatg tcaaaaaata ctttcggtct
961 tagctccatt agtgccaaca ggctcagaaa atttaaaaag cctttataat actgtctgcg
1021 tcatctggtg cattcacgca gaagagaaag tgaaacacac tgaggaagca aaacagatag
1081 tgcagagaca cctagtggtg gaaacaggaa cagcagaaac tatgccaaaa acaagtagac
1141 caacagcacc atctagcggc agaggaggaa attacccagt acaacaaata ggtggtaact
1201 atgtccacct gccattaagc ccgagaacat taaatgcctg ggtaaaattg atagaggaaa
1261 agaaatttgg agcagaagta gtgccaggat ttcaggcact gtcagaaggc tgcaccccct
1321 atgacattaa tcagatgtta aattgtgtgg gagaccatca agcggctatg cagattatca
1381 gagatattat aaatgaggag gctgcagatt gggacttgca gcacccacaa ccagctccac
1441 aacaaggaca gcttagggag ccgtcaggat cagatattgc aggaacaact agttcagtag
1501 atgaacaaat ccagtggatg tacagacaac agaaccccat accagtaggc aacatttaca
1561 ggagatggat ccaactgggg ttgcaaaaat gtgtcagaat gtataaccca acaaacattc
1621 tagatgtaaa acaagggcca aaagagccat ttcagagcta tgtagacagg ttctacaaaa
1681 gcttaagagc agaacaaaca gatgcagcag taaagaattg gatgactcaa acactgctga
1741 ttcaaaatgc taacccagat tgcaagctag tgctgaaggg gctgggtgtg aatcccaccc
1801 tagaagaaat gctgacggct tgtcaaggag tagggggacc aggacagaag gctagattaa
1861 tggcagaagc cctgaaagag gccctcgcac cagtgccaat cccttttgca gcagcccaga
1921 agaggggacc aagaaagcca attaagtgtt ggaattgtgg gaaggaggga cactctgcaa
1981 ggcaatgcag agccccaaga agacagggat gctggaaatg tggaaaaatg gaccatgtta
2041 tggccaaatg cccagacaga caggcgggtt ttttaggcct tggtccatgg ggaaagaagc
2101 cccgcaattt ccccatggct caagtgcatc aggggctgac gccaactgct cccccagagg
2161 acccagctgt ggatctgcta aagaactaca tgcagttggg caagcagcag agagaaagca
2221 gagagaagcc ttacaaggag gtgacagagg atttgctgca cctcaattct ctctttggag
2281 gagaccagta gtcactgctc atattgaagg acagcctgta gaagtattat tggatacagg
2341 ggctgatgat tctattgtaa caggaataga gttaggtcca cattataccc caaaaatagt
2401 aggaggaata ggaggtttta ttaatactaa agaatacaaa aatgtaaaaa tagaagtttt
2461 aggcaaaagg attaaaggga caatcatgac aggggacact ccgattaaca tttttggtag
2521 gaatttgcta acagctctgg ggatgtctct aaatcttccc atagctaagg tagagcctgt
2581 aaaagtcacc ttaaagccag gaaaggttgg accaaaattg aagcagtggc cattatcaaa
2641 agaaaagata gttgcattaa gagaaatctg tgaaaagatg gaaaaggatg gtcagttgga
2701 ggaagctccc ccgaccaatc catacaacac ccccacattt gccataaaga aaaaagataa
2761 gaacaaatgg agaatgctga tagattttag ggaactaaat agggtcactc aggactttac
2821 agaagtccaa ttaggaatac cacaccctgc aggactagca aaaaggaaaa ggattacagt
2881 actggatata ggtgatgcat atttctccat acctctagat gaagaattta ggcagtacac
2941 tgcctttact ttaccatcag taaataatgc agagccagga aaacgataca tttataaggt
3001 tctgcctcag ggatggaagg ggtcaccagc catcttccaa tacactatga gacatgtgct
3061 agaacccttc aggaaggcaa atccagatgt gaccttagtc cagtatatgg atgacatctt
3121 aatagctagt gacaggacag acctggaaca tgacagggta gttttacagc taaaggaact
3181 cttaaatagc atagggttct ctaccccaga agagaaattc caaaaagatc ccccatttca
3241 atggatgggg tacgaattgt ggccgacaaa atggaagttg caaaagatag agttgccaca
3301 aagagagacc tggacagtga atgatataca gaagttagta ggagtattaa attgggcagc
3361 tcaaatttat ccaggtataa aaaccaaaca tctctgtagg ttaattagag gaaaaatgac
3421 tctaacagag gaagttcagt ggactgagat ggcagaagca gaatatgagg aaaataagat
3481 aattctcagt caggaacaag aaggatgtta ttaccaagaa ggcaagccat tagaagccac
3541 ggtaataaag agtcaggaca atcagtggtc ttataaaatt caccaagaag acaaaatact
3601 gaaagtagga aaatttgcaa agataaagaa tacacatacc aatggagtta gactattagc
3661 acatgtaata cagaaaatag gaaaggaagc aatagtgatc tggggacagg tcccaaaatt
3721 ccacttacca gttgagaggg atgtatggga acagtggtgg acagactatt ggcaggtaac
3781 ctggataccg gagtgggatt ttatctcaac gccaccacta gtaagattag tcttcaatct
3841 agtgaaggac cctatagagg gagaagaaac ctattataca gatggatcat gtaataaaca
3901 gtcaaaagaa gggaaagcag gatatatcac agataggggc aaagacaaag taaaagtgtt
3961 agaacagact actaatcaac aagcagaatt agaagcattt ctcatggcat tgacagactc
4021 agggccaaag acaaatatta tagtagattc acaatatgtt atgggaataa taacaggatg
4081 ccctacagaa tcagagagca ggctagttaa ccaaataata gaagaaatga ttaaaaagtc
4141 agaaatttat gtagcatggg taccagcaca caaaggtata ggaggaaacc aagaaataga
4201 ccacctagtt agtcagggga ttagacaagt tctcttcttg gaaaagatag agccagcaca
4261 agaagaacat gataaatacc atagtaatgt aaaagaattg gtattcaaat ttggattacc
4321 cagaatagtg gccagacaga tagtagacac ctgtgataaa tgtcatcaga aaggagaagc
4381 tatacatggg caggtaaatt cagatctagg gacttggcaa atggactgta cccatctaga
4441 aggaaaaata gtcatagttg cagtacatgt agctagtgga ttcatagaag cagaagtaat
4501 tccacaagag acaggaagac agacagcact atttctgtta aaattggcag gcagatggcc
4561 tattacacat ctacacacag ataatggtgc taactttgcc tcgcaagaag taaagatggt
4621 tgcatggtgg gcagggatag agcacacctt tggggtacca tacaatccac agagtcaggg
4681 agtagtggaa gcaatgaatc accacctgaa aaatcaaata gatagaatca gggaacaagc
4741 aaattcagta gaaaccatag tattaatggc agttcattgc atgaatttta aaagaagggg
4801 aggaataggg gatatgactc cagcagaaag attaattaac atgatcacta cagaacaaga
4861 aatacaattt caacaatcaa aaaactcaaa atttaaaaat tttcgggtct attacagaga
4921 aggcagagat caactgtgga agggacccgg tgagctattg tggaaagggg aaggagcagt
4981 catcttaaag gtagggacag acattaaggt agtacccaga agaaaggcta aaattatcaa
5041 agattacgga ggaggaaaag aggtggatag cagttcccac atggaggata ccggagaggc
5101 tagagaggtg gcatagcctc ataaaatatc tgaaatagaa aactaaagat ctacaaaagg
5161 tttgctatgt gccccatttt aaggtcggat gggcatggtg gacctgcagc agagtaatct
5221 tccccctaca ggaaggaagc catttagaag tacaagggta ttggcatttg acaccagaaa
5281 gagggtggct cagtacttat gcagtgagga taacatggta ctcaaggaac ttttggacag
5341 atgtaacacc agactatgca gacattttac tgcatagcac ttatttccct tgctttacag
5401 cgggagaagt gagaagggcc atcaggggag aacaactgct gtcttgctgc aagttcccga
5461 gagcacatag gtaccaggta ccaagcctac agtacttagc actaaaagta gtaagcgatg
5521 tcagatccca gggagagaat cccacctgga aacagtggag aagagacaat aggagaagcc
5581 ttcgaatggc taaacagaac agtagaggag ataaacagag aggcagtaaa ccacctacca
5641 agggagctga ttttccaggt ttggcaaagg tcttgggaat actggcatga tgaacaaggg
5701 atgtcacaaa gctatgtaaa atacagatac ttgtgtttaa tgcaaaaggc tttatttatg
5761 cattgcaaga aaggctgtag atgtctaggg gaaggacacg gggcaggagg atggagacca
5821 ggacctcctc ctcctccccc tccaggacta gcataatagg aagaaagacc tccggaaaat
5881 gaaggcccac aaagggaacc atgggatgaa tgggtagtgg aggtttagga agaattgaaa
5941 gaagaagctt taaaacattt tgatcctcgc ttgctaactg cacttggtaa tcatatctat
6001 aatagacatg gagacaccct tgagggagca ggagaactca ttagaatcct ccaacgagcg
6061 ctcttcatgc attttagagg cggatgcaac cactccagaa tcggccaacc tgggggagga
6121 aatcctctct caactatacc gccctcttga ggcgtgctat aacacatgct attgtaaaaa
6181 gtgttgctac cattgccagt tttgttttct taaaaaggga ttggggatat gttatgagca
6241 gtcacgaaag agaagaagaa ctccgaaaaa ggctaaggct aatacatctt ctgcatcaaa
6301 caagtaagta tgccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
6361 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagtatcac catgagtgca
6421 gaggtggcag aactgtatcg attggagttg ggagattata aattagtaga gatcactccg
6481 attggcttgg cccccacaga tgtgaagagg tacactactg gtggcacctc aagaaataaa
6541 agaggggtct ttgtgctagg gttcttgggt tttctcgcaa cggcaggttc tgcaatgggc
6601 gcggcgtcgt tgacgctgac cgctcagtcc cggactttat tggctgggat agtgcagcaa
6661 cagcaacagc tgttggacgt ggtcaagaga caacaagaat tgttgcgact gaccgtctgg
6721 ggaacaaaga acctccagac tagggtcact gccatcgaga agtacttaaa ggaccaggcg
6781 cagctaaatg cttggggatg tgcgtttaga caagtctgcc acactactgt accatggcca
6841 aatgcaagtc taacaccaga ctggaacaat gatacttggc aagagtggga gcgaaaggtt
6901 gacttcttgg aggaaaatat aacagccctc ctagaagagg cacaaattca acaagagaag
6961 aacatgtatg aattacaaaa gttgaatagc tgggatgtgt ttggcaattg gtttgacctt
7021 gcttcttgga taaagtatat acaatatgga atttatgtag ttgtaggagt aatactgtta
7081 agaatagtga tctatatagt acaaatgcta gctaagttaa ggcaggggta taggccagtg
7141 ttctcttccc caccctctta tttccagtag actcataccc aacaggaccc ggcactgcca
7201 accagagaag gcaaagaagg agacggtgga gaaggcggtg gcaacagctc ctggccttgg
7261 cagatagaat atattcattt cctgatccgc caactgatac gcctcttgac ttggctattc
7321 agcaactgca gaaccttgct atcgagagca taccagatcc tccaaccaat actccagagg
7381 ctctctgcga ccctacgaag ggttcgagaa gtcctcagga ctgaactgac ctacctacaa
7441 tatgggtgga gctatttcca tgaggcggtc caagccggct ggagatctgc gacagaaact
7501 cttgcgggcg cgtggagaga cttatgggag actcttagga gaggtggaag atggatcctc
7561 gcaatcccta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
7621 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
7681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
7741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
7801 tcatgtctgg atcgaattcg atatcatcat tgggacgtca ggtggcactt ttcggggaaa
7861 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
7921 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
7981 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
8041 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
8101 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
8161 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
8221 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
8281 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
8341 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
8401 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
8461 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
8521 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
8581 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
8641 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
8701 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
8761 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
8821 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
8881 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
8941 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
9001 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
9061 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
9121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
9181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
9241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
9301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
9361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
9421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
9481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
9541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
9601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gggccctagg
9661 ccggcctcgg ccgctctaga actagtggat ccgggccgct ctagagagct tggcccattg
9721 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
9781 ccatgtt
//
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